Welcome to the Web site of the lab of Timothy A. Ryan, Ph.D. in
the Department of Biochemistry at the
The lab participates the following programs:
Physical Chemistry of the Synapse
The focus of this lab is the study of the molecular basis of synaptic transmission in the mammalian brain. Our primary interests lie in understanding the regulation of vesicle traffic in presynaptic terminals, and how this traffic impacts presynaptic function.
We use biophysical tools to measure vesicle trafficking events in individudal presynaptic boutons and molecular, chemical and genetic tools to investigate the role of specific proteins in these events. We concentrate heavily on state-of-the-art optical methods to obtain a quantitative understanding of presynaptic function and attempt to reduce the complexities of synaptic transmission in a physico-chemical framework.
ã T.A. Ryan 2004 all rights reserved.
GFP labeled dendrites and axons in living SNAREs during recycling imaged synataxin Ia distribution in
hippocampal neurons in culture (green) ith synapto-pHluorin (green) living cells vizualized with pHluorin
Synaptic vesicle clusters visualized using FM 4-64 (red)
GFP-synapsin Ia distribution Recycling vesicle clusters in synaptotagmin I KO synaptic terminals
This page was last updated on 11/17/05.